Fig 1: The effect of rhPRG4 on FAT10 expression. (A) FAT10’s role in NF?B signaling. Created with BioRender.com (B) Heatmap showing genes that were most significantly changed by TNFa and rhPRG4. (C) FAT10 RNA expression in RPM from RNA sequencing (N = 3, assessed by one-way ANOVA, * p < 0.05, ** p < 0.01). (D) FAT10 expression on the protein level by Western Blotting. (E) Relative FAT10 expression normalized to GAPDH expression and to CTRL samples, analyzed using GeneTools software (N = 5–7, assessed by two-way t-tests with Bonferroni correction, * p < 0.05, *** p < 0.001).